Results - Cross. Fit 9. In August of 2. 01. Melissa Swanson, asked me if I wanted to go with her to Cross. Fit 9. 06 on a “Bring a Friend” Saturday. Now, over a year later, I am using a blue band for pull ups and can do 2. I’m not done with my goals but I will get there with my WOD partner and hubby John. We were sitting at work one day, five years ago, bemoaning how fat we were. I was working out at the Y, not a lot, but some and Shay told me how he had just started exercising with Jill doing this “crossfit” workout. Well you know what happened to Shay (poor guy, losing all that weight). What happened to me, not much! I would lose a little, gain it back, up and down. Exercise some, sometimes not. When I would ask, Shay would talk about the diet and the exercising. He encouraged me to join him. I did a couple of times, but I’m not a natural gym rat. I’m a really good couch potato. He lent me his copy of the zone diet. The one thing going for me was I had been slowly, for years, changing my diet for the better. After reading about the zone diet, I tweaked my diet some more. But still no serious exercise. When Shay and Jill opened up the closet, (it’s hard to call the first Box a Box, maybe shoebox), Shay wanted me to join; I think he was tired of hearing me whine. Again he invited me over, and I would do some workouts, but only a few. I told him when he opened the bigger one, I would join. They opened the bigger box and I finally joined. Shannon and Nicole were great getting me through the on- ramp. It took some time before I got in a good rhythm of working out around my shift work and I still run into times were it is difficult to get in a workout. In the first year or so, my gaps were long, month or two at a time. So no noticeable change, though each time I came back I realized I didn’t lose much strength, just endurance. Here you will find a list of Do’s & Don’ts for eating on a strict Paleo Diet. Lose weight, increase your fitness, and have more energy. The Zone was created by Barry Sears, Ph.D. Sears's philosophy is that food can affect the hormonal response of the body and cause changes in insulin production. Finally, last January, I made a resolution to not let the gaps be long. I weighed 1. 80, my 3. I was still on a black band doing pull- ups. I was winded doing any running, and could barely bench 1. I set a goal of bench pressing my body- weight, no band kipping pull ups and “Fran” Rx’d in under 1. I nearly passed out half way through, but did finish it after resting for about 2. Nine months later, I lost the bands. I recently reached a new bench press PR of 1. Just a couple of weeks ago, “Fran” was the workout. I Rx’d it but it took 1. I am on my way to reaching those goals. Also I measured my waist early last year and it was 4. Megan measured me and I was down to 3. The belly is shrinking, the weight is below 1. I feel great. My favorite time to work out is in the morning with the ladies. If you ever work out with Laurie and/or Vanda, you have no room to whine. I am more inspired watching those two give there all during a WOD than anyone I have ever watched. Nicole, Becky and Tanya ensure I am not going too light, always making sure I am properly pushing the limit as we scale the WOD’s. I’ve worked out with Shay only a handful of times. Does the Zone Diet work? Dietitian Juliette Kellow investigates the zone. Julianne's Paleo & Zone Nutrition Nutrition stuff I find interesting with a Paleo Zone flavour by New Zealand nutritionist Julianne Taylor. ZONE Food Blocks Here is a guide on Zone Food Blocks to help you choose the right amount of foods to keep you In the Zone. The Zone diet is primarily concerned with controlling your hormones. Hormonal balance affects all important components of your wellness: body composition, energy. Paleo Diet Food List The following is a comprehensive paleo diet food list. I wonder how much effort he puts in, because I think he just watches me do my rounds, encouraging me and then after, fine tuning my technique. He is a nut about form. What I like about crossfit is not having to figure out what workout I’m going to do and workouts that don’t take a lot of time. At the Box, you can get in, the WOD is on the board, and get it done. If you want to start before the group, no problem, they will cheer you on, while they warm up. Everyone (without exception) encourages, never discourages; I never heard anyone at other gyms cheer you on. I have finally found a gym I WANT to go to.
I’m finally getting results which continues to encourage me to go. I encourage anyone who wants to lose weight and gain strength, to join. Thank you Jill and Shay for opening Crossfit 9. Bill. John H. HARD WORK + CONSISTENCY + FUN = RESULTSSeveral years ago my wife, Jaime, joined Cross. Fit and since day one she told me I had to join. All she talked about was “Cross. Fit this” and “Cross. Fit that”. I told her there was no way I was EVER going to join the Cross. Fit “cult“! Well, on April 1. I surrendered to pressure and joined Cross. Fit 9. 06. Oh Boy! Starting out at a weight of 3. I learned later that the workout was just a baseline which confirmed how out of shape I really was. From that day on, I promised myself I would consistently follow the Cross. Fit program from on- ramp to working out – 3 days on, one day off. I knew I was not the fastest or strongest guy but I would work hard at a safe pace and push myself to get stronger. During this journey I found my new favorite word – “REALLY!!!???” “Lift what over my head how many times? Really?” “Hang from a bar and touch my feet to my elbows? Really?” Well, one year later I am 7. I am much stronger and truly believe I have a shot at being in better shape when I hit age 5. I was at 1. 8. It turns out that what I jokingly referred to as a cult has become an extended family to me. I have never felt more at home than when I am at the box. My Cross. Fit 9. 06 family is the most encouraging, motivating, friendly and competitive group of individuals I have ever met. If you’re looking for a way to improve your health and have fun, try Cross. Fit 9. 06.- John (Bear) Harrington. Ross. Jolene. I started my weight loss journey about 3 months before I joined CF. I had lost 2. 0 pounds walking/jogging on my treadmill, but I was getting bored. I had to think of something else to do or else I was going to fail. I was out with the family running some errands and have driven by Cross. Fit 9. 06 many times, this time I asked if we could stop so I could check it out. By chance, Jill was there that afternoon and she set up a consult for the next day. I had no idea what I was in for. She told me that it would take 5 to 7 minutes to complete; just over 7 minutes later I had completed half. When leaving Cross. Fit 9. 06 I knew that this was something I wanted to try, even after the days following my baseline and being so sore I looked forward to starting on- ramp. On November 2. 6, 2. I became a member at Cross. Fit 9. 06. I have strengthened my back, hips and knees and no longer suffer the pain I did on a daily basis. My goal when becoming a Crossfitter was to lose weight and gain muscle. I have done both and so much more, I have lost a total of 3. I have begun to see definition in my arms, legs and my back. I have increased my confidence and lowered my feelings of depression. I look forward to my morning workouts; I have seen myself progress and become capable of things I never thought I could do. I am dedicated to making this work, it is hard work but it has paid off beyond what I have expected. When asked if I would do a Bio for Cross. Fit 9. 06 my first reaction was “NO WAY, I cannot put myself out there!” however if I could help one person to get where I am today I know it will be worth it. I have learned that the voice in my head is a liar and will tell me I cannot do it long before my body fails. I’ve taught myself to listen to my body and not that voice. I would like to end with saying that as a shy person involved closely with my family (wife and mother of 4) this was out of the box for me to try. I have overcome many obstacles and made a few accomplishments as well, my newest goal is to complete a 5. K by the end of summer. If you’re thinking about trying this out, I say get started now. I knew I was a little over weight (if you consider 5. However, it wasn’t just the weight that concerned me, these recent results showed my blood pressure and cholesterol were high too. If I didn’t do something now then I would be on medication and I was way too young for that to be my option. Not knowing how I got to this point (as I was always active in high school and college) I knew I needed to do something not only for me but for my six month old son (my pride and joy). And thus my fitness journey began. I knew I needed to find a gym, preferably one that was open 2. I had feebled attempts in the past at these globo sorts of gyms, only to have my motivation lost after the first couple of weeks. I knew this time; I had to find something different. Then a friend mentioned Cross. Fit 9. 06. And of course off to their website I went. I was immediately intrigued and thought just maybe this could be what I needed. So, I got on the phone and scheduled my consultation for January 1. Not sure what to expect I walked in ready to go, or so I thought! I got the tour from Shawn, filled out my waiver (for the timed baseline). I felt pretty confident and thought I got this. After the baseline, I could hardly walk, breath, sit and how I managed to drive home is still a mystery to me. As I lay on my couch that night, I couldn’t believe that after only 6 minutes and 4. I could be this beat. But ironically it was a great feeling and I was hooked. I went back the next day to start my On Ramp and the rest is history. It sure has been a journey these past two years. I have missed days here and there and even a week or two only to find myself pissed as you get that baseline soreness all over again. But, it is all worth it. I have lost nearly 3. And most important I feel awesome. My wife joined shortly after I did and now my son enjoys going to the box as well. We can’t wait for him to start Cross. Fit Kids this summer. I just want to say thank you to everybody I have met at Cross. Fit 9. 06. There are too many names to list from the girls in the morning, who do not like my taste in music, to all the trainers you guys are amazing, and to all my fellow athletes thank you!!! And of course to Shay and Jill, if it weren’t for you none of this would be possible. You guys are truly amazing and thank you so much for bringing your passion of Cross. Fit to the rest of us. I am so proud to be part of the Cross. Fit 9. 06 family and so grateful for the lifestyle change it has given me!!— Chris Sgouris. Shay. July 2. 01. Shay was 2. 5 years into Cross. Fit. The photo on the right is current Shay’s progress over the years- “Filthy Fifty”7- 1. Rx’d“Jackie”3- 6- 0. Blue band pull- ups Feb.
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How to Gain Muscle Fast (with Pictures)2. Focus exclusively on weight- training. You might enjoy other cardio activities (sports, running, etc.) but those put additional strain on your body (muscles, joints) and use up energy that could be used instead to build muscle. Normally, cardio activities should definitely be included for all- around health, but if you're temporarily focused on building muscle quickly, focusing on weight- training exclusively for a few months will help you reach that goal. Build a Powerful Chest and Punch Without Weights . There’s something about well- built pecs that screams “Warrior!” Or maybe it’s that a thick chest makes you more likely to reflect a lone spear that’s flying through the air? Beats me, but one thing’s for sure: all guys want a big chest, and all women want a guy with impressively powerful pecs. It’s hard- wired into our DNA. Research shows that women are more sexually attracted to men who have who a low waist- to- chest ratio. You know, that V- shaped look. Total-body workouts How to build muscle and strength without lifting weights Give yourself a break from all the heavy lifting and reap the muscle-building benefits of. Strength training is a type of physical exercise specializing in the use of resistance to induce muscular contraction which builds the strength, anaerobic endurance. So you wanna put on some lean muscle mass. And you want to do it within the context of the Primal Blueprint, but aren’t sure where to start. And yes, research was actually performed and money was spent to come to this blatantly obvious conclusion. The cool thing about building a more powerful chest is that it can be done remarkably well without any equipment whatsoever. I’ll tell you right now that adding meat to your chest doesn’t require much ingenuity. Start doing 1. 00 push- ups every day and you’ll get the job done. However, there are two shortcomings with this approach. First, you won’t build a powerful chest that way. Developing muscular power to elite levels requires a more explosive approach. High volume push- up training develops muscular endurance without regard for power. Since the pecs respond well to sets of high reps, doing 1. Second, it’s likely that the length- tension relationship around your shoulder joint will regress when you do nothing but traditional push- ups all the time. Most men who can't gain muscle weight are eating and exercising the wrong way. Here are 10 principles to pack on as much as a pound of muscle each week.At the very least, it will magnify underlying shoulder dysfunction. This leads to pain that eventually makes you stop all chest work, and damn it if you’re not back to where you started. So I’ve put together a plan that will supercharge your pecs, improve shoulder health, and build knockout power at the same time. Warm- up the Shoulders: arm circles. Preparing for chest training couldn’t be any simpler. Start with 2. 0 arm circles in each direction. The circles should start small and get progressively larger every few reps. By the time you reach rep 1. Also, do these arm circles faster and faster over time. Your own bodyweight, everyday objects and resistance bands all help you build strength in your chest, abs and biceps without heavy free. Intermittent fasting diet for fat loss, muscle gain and health. Articles, research, diet advice, and free guides from IF-expert, Martin Berkhan. Don’t rip into 2. Activate the Core: side plank with rotation. Before my clients do any chest training, I always have them perform the side plank with rotation. This exercise activates the lats and side core muscles which immediately enhances your upper body pushing power. If you’re ever in a bar with one of those punching bag machines that measures the power of your punch and your buddy bets you $5. When you return, he won’t suspect a thing and you’re sure to beat him. Well, unless he’s Shane Carwin. In that case you’re screwed. Perform one set of 1. This exercise should be performed slowly with maximum core tension throughout the movement. Build Shoulder Stability Strength: hand over hand. Once the core and lats have been activated it’s not time to jump into any explosive training just yet. To protect the shoulder joints it’s important to activate and strengthen the rotator cuff while boosting stability in the glenohumeral joint. The hand over hand exercise gets the job done. Perform one set of five holds on each side (1. Hold the position for 1- 2 seconds each time before switching sides. Explosive Power: 3 Position Hand Hop. Now it’s time to turn up the juice with an exercise I use to build explosive power in the chest and core. The 3 position hand hop is an outstanding exercise for fighters because it trains your chest to fire with the core. When you jump your hands out to different positions, your core has to perform a powerful contraction to stabilize your torso. Each set should be performed with maximum speed. Perform three reps as fast as possible. You’ll jump your hands out to three different positions and back to the center for one rep. Perform five sets of three reps with 3. This exercise is ideal for building explosive punching power. For fighters who need more shoulder endurance, perform as many reps as possible before your technique breaks down as an alternative to just three reps. This approach is also excellent for building muscle. Hypertrophy and Endurance: push- up (optional)At this point you have two choices. You can stop here if your chest is already smoked from the hand hop, and if chest power is your primary goal. Or, if you really need more chest mass this is the perfect time to knockout two sets of as many reps as possible with the good ol’ fashioned push- up. Use a hand position that’s shoulder width, or slightly wider, and use a variation that allows 2. For most guys this will be a regular push- up. However, if you can do more than 3. Swiss ball. For the second set, repeat the same exercise and do as many reps as possible, even if it’s not 2. The Plan. Perform the following routine every other day for four weeks. Put this at the beginning of your workout on the days you perform your regular training. Arm circles for 2. Side plank with rotation for 1. Hand over hand for 5 holds on each side. Push- up for 2 sets of AMRAP, rest 6. World Wise Schools promotes the Peace Corps' Third Goal of teaching Americans about the world by providing educational resources to promote global competence.The Complete Low- FODMAP Diet . The low- FODMAP diet is the long- awaited answer. In clinical trials, over three quarters of people with chronic digestive symptoms gain significant relief by reducing their intake of FODMAPs—difficult- to- digest carbs found in foods such as wheat, milk, beans, soy, and certain fruits, vegetables, nuts, and sweeteners. 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Best Slimming Foods - Weight Center. Looking for a miracle diet food? It's time to call off the search — there's no such thing. While they're not miracle foods, they can help you eat less over the course of the day. This portable fruit is the perfect snack, with a high water content and both kinds of weight- busting fiber: soluble, which helps prevent blood sugar spikes that lead to cravings, and insoluble, which helps fill you up. To sweeten the pot, apples also contain quercetin, a flavonoid shown to help fight certain cancers, reduce cholesterol damage and promote healthy lungs. TIP: Organic apples are worth the extra cost because commercial apples retain more pesticide residue than fruits you peel, such as oranges or bananas. And with half the fiber and most of the iron, magnesium and vitamin C, you definitely want to eat that peel to get the full slimming benefits. Eggs. They're a great source of protein, which may be key to keeping you full. A recent study published in the Journal of the American College of Nutrition found that when people ate two eggs for breakfast, they took in more than 4. Dhurandhar, Ph. D., from Pennington Biomedical Research Center in Baton Rouge, Louisiana. Although this was only a one- day study, eating eggs regularly may have long- term weight management benefits, says Dr. Dhurandhar. TIP: For a healthier egg, farmers are improving the hen feed by adding canola oil, alfalfa, rice bran and even sea kelp. Try Eggland's Best eggs, which contain omega- 3 fatty acids, along with more vitamin E and less saturated fat than regular eggs. One study found that eating a breakfast of two eggs can leave you feeling full longer than a bagel. Cauliflower. It's good for you, too. Cauliflower contains the cancer- fighting phytonutrient sulforaphane, as well as a good amount of folate and vitamin C, which may be helpful for weight loss. In fact, a review from Purdue University pointed to vitamin C status as a key factor in how much fat is burned during physical activity. All that and it's pretty tasty, too. Steam a head of cauliflower and mash it with garlic salt, a sprinkling of grated Parmesan cheese and a touch of butter. Lowfat Yogurt. If the yogurt ads are to be believed, you should be fitting into that itty- bitty bikini before you know it. While yogurt and other dairy products are not weight- loss magic bullets, there is some truth in advertising. A recent study at the University of Tennessee found that dieters eating three servings of yogurt daily lost twice as much weight as their non–dairy– eating counterparts on a 1. Zemel, M. D., a professor of nutrition and medicine at the University of Tennessee in Knoxville. No word yet on how effective ice cream is at burning fat, but we're not holding our breath. TIP: Sneak more dairy into your diet by adding plain yogurt to dips, sauces and salad dressings. You get the health benefits without the added sugar of flavored yogurts. Oatmeal. Mom didn't know how right she was: Breakfast is the most important meal of the day — just eating it can make you slimmer. Researchers at the University of California, Berkeley analyzed a national six- year survey and found that people who ate breakfast had a lower body mass index (BMI) than people who skipped breakfast, and that those who ate cooked cereal had a lower BMI than any other breakfast- eating group. Also, oatmeal was ranked as the most satiating breakfast food on the Satiety Index, developed by Australian researchers a decade ago, and it's the third most satiating food overall. Katz. TIP: If you don't have time for cooked oatmeal every morning, make muesli by mixing old- fashioned oats with plain yogurt, dried fruit and fruit juice and leaving it in the fridge overnight. Peanuts. Almonds and walnuts have been getting all the glory these days, but don't discredit peanuts, the most commonly eaten nut in America. Researchers at Purdue University found that peanut eaters end up eating less over the course of the whole day and are more likely to maintain weight, even if given as many peanuts as they want. So what is it about peanuts? Turns out it's a little bit of everything. A recent study published in the journal Obesity Research found that adding two 1. Adding water into a food makes it more filling than drinking water separately. Rolls, lead researcher on the study. The Australian Satiety Index ranks steamed white fish such as halibut or cod as the number- one most filling food out of 3. Also, a new study from Karolinska Institutet in Sweden found that people ate 1. Katz. TIP: To add some flavor to grilled fish, try a quick marinade of soy sauce, lime and ginger. Bulgur. High- fiber grains are a great way to round out a meal, and fine- cut bulgur is easy to cook. Bulgur, which is a quick- cooking form of whole wheat, takes about 1. With all the good fiber comes some other benefits: iron and vitamins E and B6. TIP: Find bulgur in health- food stores and organic markets. For a quick side dish, combine fine bulgur with chicken broth, diced canned tomatoes and some cooked onions. Can you give me some tips to losing weight? Answer; Where can I find a dietitian who works with people who want to lose weight? Answer; I have been trying to find out. Uses, Benefits, Cures, Side Effects, Nutrients in Black Salt. List of various diseases cured by Black Salt. How Black Salt is effective for various diseases is listed. Chiku is rich in dietary fibre, may be used as a good natural laxative. Being having adequate fibre, it helps to aid in digestion thus relieves from indigestion and. Salad. When most people think of dieting, they think of salad. 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Bulgur doesn't lose much from its minimal processing; it remains high in protein and minerals. That means it's an ideal foundation for meals, allowing you to skip higher- fat protein sources, like most meats. Bulgur is also a standout in terms of its fiber content, just like whole wheat, and can help keep your digestive tract healthy as a result. The insoluble fiber it contains absorbs water, promoting faster elimination of waste, which prevents the formation of an environment that promotes the development of carcinogens. Selection and Storage. You may need to visit a health- food store to find bulgur. It's available in three grinds - - coarse, medium, and fine. Coarse bulgur is used to make pilaf or stuffing. Medium- grind bulgur is used in cereals. The finest grind of bulgur is suited to the popular cold Middle Eastern salad called tabbouleh. Store bulgur in a screw- top glass jar in the refrigerator; it will keep for months. Preparation and Serving Tips. Because bulgur is already partially cooked, little time is needed for preparation: Combine a half cup of bulgur with one cup of liquid and simmer for 1. Let stand for another ten minutes. For cold salads, soak bulgur before using: Pour boiling water over bulgur, in a three- to- one ratio. Soak for 3. 0 to 4. Drain away excess water. If you like your bulgur chewier, let it sit longer to absorb more water. Bulgur is used like rice in Mediterranean countries. In fact, you can use bulgur in place of rice in most recipes. Bulgur lends its nutty flavor to whatever it is combined with, allowing you to use it as a main ingredient, thus cutting back on higher- calorie foods. Another health bonus, bulgur has more fiber than oats, buckwheat, or corn. Plus, its quick cooking time and mild flavor make it ideal for those new to whole grain cooking. Its ability to fill you up with few calories is great for weight- loss dieters. Ways to Tone Your Body. Whether you want to tone up after an indulgent. 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PUBLISHED: November 19, 2016 08:00 Weight-loss surgery centre being planned for Shrewsbury or Telford hospitals. The number of weight-loss surgery procedures is set. Find healthy eating, fitness and diet tools for healthy weight loss. All rights reserved. Boots. Web. MD does not provide medical advice, diagnosis or treatment. See additional information. The Surgical Weight Loss Centre. We’ve moved location! We’ve expanded into new, larger premises. Don’t worry, we haven’t moved far. We’re still located at Pindara Hospital, just in the building next door to our old address. Our new rooms are located at Suite 1. Level 2) Pindara Place, 1. Carrara St, BENOWA Q 4. We host free information evenings so you can hear the facts from. Meningitis B Vaccination Programme The Riverside Surgery offers the Meningitis B vaccine to babies alongside other routine vaccinations at 2 months, 4 months, and 12. How One Woman Shed 137 Lbs. With Strength Training and Simple Diet Changes. This is how easy it is. Click here to find out how many calories you use in a day – just sitting on your butt, you probably use anywhere from 2500. Weight Loss & Obesity Overview. Being obese means having so much body fat that your health is in danger. Having too much body fat can lead to type 2 diabetes, heart. Eating less and moving more are the basics of weight loss that lasts. For some people, prescription weight loss drugs may help. You'll still need to focus on diet and. You can usually leave hospital one to three days after weight loss surgery and start to return to your normal activities four to six weeks later. Life after weight loss surgery will bring weight loss and health benefits along with significant diet and lifestyle changes and challenges. Oral treatment with Eubacterium hallii improves insulin sensitivity in db/db mice : npj Biofilms and Microbiomes. E. Cultures were grown to the end of the exponential phase, concentrated by anaerobic centrifugation, washed with phosphate- buffered saline, diluted in a solution containing maltodextrin and glucose in 1. CFU), 1. 08 CFU and 1. CFU in 1. 00 . Viability was assessed by most probable number analysis by dilution to extinction and confirmed by microscopic analysis. Samples were stored at . Male C5. 7Bl. 6/J db/db mice (1. Jackson Laboratories USA. Animals were housed at AMC SPF vivarium in groups of 5 animals/cage and fed ad libitum with regular chow diet (Research Diets, Inc.) and water. Mice were housed under constant temperature and a 1. At 1. 6 weeks of age, the animals were daily given an oral 1. As a control, an oral 1. Twenty- four- hour faeces were collected after 4 weeks of treatment (2. In the last week of treatment and after an overnight fast, mice (n=8 per group) received an intraperitoneal insulin bolus (Actrapid 0. U/kg body weight) and blood glucose was measured (Ascensia Elite glucose meter, Bayer, Leverkusen, Germany) at t=0, 6. Thereafter, animals were sacrificed using 1. Hyperinsulinemic- euglycemic clamp. Male C5. 7Bl. 6/J6 db/db mice (1. Jackson Laboratories, Bar Harbor, ME, USA. Animals were housed at University of Gothenburg SPF vivarium and fed ad libitum with regular chow diet (Research Diets, New Brunswick, NJ, USA) and water. Marine Biofilms & Microbiomes; Facilities / Research Capacities. Overview; Sequencing Capacity; Imaging Facility;. About the npj Biofilms and Microbiomes Community. Mice were housed under constant temperature and a 1. In the last week of treatment, at least 4 days before the clamp a catheter was surgically placed in the jugular vein for infusion of insulin and glucose under isoflurane anaesthesia. Prior to the clamp, mice were fasted for 4 h and placed in individual plastic containers. Basal blood glucose (Countour Next blood glucose meter, Bayer AB, Solna, Sweden) was used from tail- blood measurements. A bolus injection of . Three consecutive blood samples were taken at steady state (t=. At t=0, a priming dose of insulin (1. U/kg; Actrapid Penfill, Novo Nordisk, Bagsv. Blood glucose was measured at 1. GIR; 3. 0% glucose Fresenius Kabi, Bad Homburg, Germany) to maintain blood glucose concentration at the basal level. At steady state, defined by stable glycemia and GIR (approximately at t=1. Rd) and hepatic glucose production (Ra) under hyperinsulinemic- euglycemic- conditions. Plasma insulin was measured at t=. Animals were killed by an overdose of pentobarbital (Apoteket Farmaci AB, Stockholm, Sweden) and tissue was collected. The blood samples were deproteinised, evaporated and resuspended in deionised water for the determination of radioactivity (Beckman LS6. Multipurpose Scintillation Counter, Providence, RI, USA). Whole- body glucose appearance (Ra) and endogenous glucose production (endogenous Ra), a measure of hepatic glucose production, were calculated as published as previously described. Metabolic chamber experiments and body composition. During a parallel experiment, male db/db mice (aged 1. Thereafter, mice were individually housed in Somedic INCA metabolic cages (Somedic AB, H. Oxygen consumption (VO2) and CO2 production (VCO2) were recorded every 2 min for 2. Temperature in the metabolic chamber was kept constant at 2. Data from the first hour was discarded to account for animal acclimatisation. The average total energy expenditure per hour was determined using Weir's equation: (3. Also, magnetic resonance imaging scanning for body composition was performed as previously described. Intestinal microbiota analysis. Abundances of E. 1. A biofilm is any group of microorganisms in which cells. S r. RNA gene amplification, in vitro transcription and labelling, and hybridisation were carried out as described. The data were normalised and analysed using a set of R- based scripts in combination with a custom- designed relational database, which operates under the My. SQL database management system. For the microbial profiling, the Robust Probabilistic Averaging signal intensities of 2. MITChip were used. Diversity calculations were performed using a microbiome R- script package (https: //github. Multivariate statistics, redundancy analysis, and principal response curves were performed in Canoco 5. Npj Aging and Mechanisms. Alzheimer Prevention, Microbes Stealing, Bacteria Hold, Health Discoveries, Alzheimers Info, Health Diet. Npj Biofilms and Microbiomes npj Biofilms. Information about the open-access journal npj Biofilms and Microbiomes in DOAJ. Introducing npj Biofilms and Microbiomes Author: Staffan Normark Subject. SCFA and bile acid profiling. Twenty- four- hour faecal samples (pooled from each cage) were collected and stored for later analysis. SFCA content was analysed by gas liquid chromatography following conversion to t- butylmethylsilyl derivate as previously described. Concentrations of different bile acids were measured twice in 2. An internal standard was added before extraction with 0. Na. OH at 8. 00 . Bile salt were trimethylsilylated with pyridine, N,O- Bis(trimethylsilyl) trifluoroacetamide and trimethylchlorosilane. Faecal bile acid profile was measured using capillary gas chromatography (Hewlett–Packert gas chromatograph; HP 6. Mountain View, CA, USA) equipped with a FID and a CP Sil 1. Plasma bile acids were determined using liquid chromatography tandem mass spectrometry as described previously (1. The primary bile acids cholic acid (CA), taurocholic acid, muricholic acid (MCA), tauroalpha muricholic acid and taurobeta muricholic acid as well as the secondary bile acids taurohyodeoxycholic acid, deoxycholic acid, taurodeoxycholic acid and omega murocholic acid were analysed in plasma and 2. The total amount of primary and secondary bile acids was calculated as the sum of the individually quantified bile salts. Quantitative real- time PCRLiver and intestinal tissues were homogenised with tissue- magna. Lyzer (Roche, Basel, Switzerland). Total RNA was extracted using Tri- pure reagent (Roche). Complementary DNA was prepared by reverse transcription of 1 . Hepatic genes involved in lipogenesis (Srebp. Fasn, Acc. 1, Acc. Dgat) and gluconeogenesis (Gck. G6. Pc, Pk and Pck. Genes involved in bile acid metabolism and transport were tested in liver (Cyp. Cyp. 8b. 1, Cyp. 7b. Cyp. 27a, Ntcp, Oatp. Mrp. 3, Bsep and Mrp. Tgr. 5, Fxr, Gata. Asbt, Ilbp Ost. Gene- specific intron–exon boundary spanning primers were used and all the results were normalised with the house keeping gene 3. B4. All samples were analysed in duplicate and data were analysed according to the 2. Primer sequences are presented in Supplementary Table S2. Statistical analysis. On the basis of distribution of the clinical data, Student t- test or Mann–Whitney tests (two- sided) were used to analyse the difference between clinical groups. Microbiota analyses were done as described above. |
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